Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia

Abstract. Aditya IGMRA, Purwanto H. 2023. Molecular detection of the pathogen Apis mellifera (Hymenoptera: Apidae) in honey Indonesia. Biodiversitas 24: 2612-2622. bees play a major role global crop pollination. The bee population faces various threats, one being entomopathogens, responsible for 13% bees' declining cases. Some pathogens are Nosema sp., Melissococcus plutonius, and Paenibacillus larvae. Data on prevalence these is not available Therefore, this study aimed to detect entomopathogen Java, Bali, Sumatra honey. We extracted 30 samples from study. DNA amplified by conventional Polymerase Chain Reaction (PCR) apis, ceranae, M. P. Amplicons were sequenced confirm species detected. Results showed that N. ceranae has 20% prevalence, found Java Bali; plutonius was, first time, Indonesia only with 3.33% prevalence. In comparison, didn't find apis Environmental factors studied their correlation incidence pathogens. From several factors, precipitation significantly correlates occurrence while other do not. This research concludes can be used as source monitoring certain area globally.